Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP.

  title={Protein interaction domains of the ubiquitin-specific protease, USP7/HAUSP.},
  author={Melissa N. Holowaty and Yi Sheng and Tin A. Nguyen and Cheryl H. Arrowsmith and Lori Frappier},
  journal={The Journal of biological chemistry},
  volume={278 48},
USP7 or HAUSP is a ubiquitin-specific protease in human cells that regulates the turnover of p53 and is bound by at least two viral proteins, the ICP0 protein of herpes simplex type 1 and the EBNA1 protein of Epstein-Barr virus. We have overexpressed and purified USP7 and shown that the purified protein is monomeric and is active for cleaving both a linear ubiquitin substrate and conjugated ubiquitin on EBNA1. Using partial proteolysis of USP7 coupled with matrix-assisted laser desorption… CONTINUE READING
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Viral plasmids in mammalian cells

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