Protein-induced fusion of phospholipid vesicles of heterogeneous sizes.

  title={Protein-induced fusion of phospholipid vesicles of heterogeneous sizes.},
  author={Beatriz Fern{\'a}ndez de Arcuri and G F Vechetti and Rosana Cheh{\'i}n and F{\'e}lix M Go{\~n}i and Roberto D. Morero},
  journal={Biochemical and biophysical research communications},
  volume={262 3},
We have investigated the fusion of phospholipid vesicles induced by lysozyme and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Vesicles were composed of dimyristoylphosphatidylcholine/dioleoylphosphatidylethanolamine/ cholesterol (DMPC:DOPE:Chol, 2:1:1). Small unilamellar vesicles (SUV, diameter ca. 30 nm) obtained by extensive sonication or large unilamellar vesicles (LUV, diameters ranged from 100 to 400 nm) obtained by extrusion methods were used. Fusion of LUV induced by lysozyme and… CONTINUE READING