Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase

@inproceedings{Kim2011ProteinII,
  title={Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase},
  author={Jee-Hyun Kim and Jun-rong Wei and Joshua B. Wallach and Rebekkah S. Robbins and Eric J. Rubin and Dirk Schnappinger},
  booktitle={Nucleic acids research},
  year={2011}
}
Using a component of the Escherichia coli protein degradation machinery, we have established a system to regulate protein stability in mycobacteria. A protein tag derived from the E. coli SsrA degradation signal did not affect several reporter proteins in wild-type Mycobacterium smegmatis or Mycobacterium tuberculosis. Expression of the adaptor protein SspB, which recognizes this modified tag and helps deliver tagged proteins to the protease ClpXP, strongly decreased the activities and protein… CONTINUE READING

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