Protein import into chloroplasts
@article{Soll2004ProteinII, title={Protein import into chloroplasts}, author={Jürgen Soll and Enrico Schleiff}, journal={Nature Reviews Molecular Cell Biology}, year={2004}, volume={5}, pages={198-208} }
Chloroplasts are organelles of endosymbiotic origin, and they transferred most of their genetic information to the host nucleus during this process. They therefore have to import more than 95% of their protein complement post-translationally from the cytosol. In vivo results from the model plant Arabidopsis thaliana — together with biochemical, biophysical and structural data from other plants — now allow us to outline the mechanistic details of the molecular machines that facilitate this…
282 Citations
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This work has shown that the secretory pathway provides new opportunities for complementation of the chloroplast protein maturation machinery with chaperones needing endoplasmic reticulum and/or Golgi typical maturations such as N-glycosylation for their biological activity or using chloroplasts as a storage compartment for glycoproteins.
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This chapter focuses on translocons located in the outer and the inner envelope membranes, and summarizes how they mediate import of nucleus-encoded proteins into the chloroplast from the surrounding cytoplasm.
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This work has shown that the biogenesis of various plastid types relies on distinct but homologous Toc–Tic import pathways that have specialized in the import of specific classes of substrates.
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Recent models and findings that help to explain important cellular mechanisms involved in the complex process of protein transport into secondary plastids are reviewed.
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- Biology
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In the present study not only the import characteristic of nine tentative ‘non-canonical’ chloroplast precursor proteins but also the new interactions between these precursor proteins and the proteins at the organellar surfaces were analyzed.
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