Protein hydrogen exchange at residue resolution by proteolytic fragmentation mass spectrometry analysis.

@article{Kan2013ProteinHE,
  title={Protein hydrogen exchange at residue resolution by proteolytic fragmentation mass spectrometry analysis.},
  author={Zhong-yuan Kan and Benjamin T Walters and Leland Mayne and S. Walter Englander},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 41},
  pages={16438-43}
}
Hydrogen exchange technology provides a uniquely powerful instrument for measuring protein structural and biophysical properties, quantitatively and in a nonperturbing way, and determining how these properties are implemented to produce protein function. A developing hydrogen exchange-mass spectrometry method (HX MS) is able to analyze large biologically important protein systems while requiring only minuscule amounts of experimental material. The major remaining deficiency of the HX MS method… CONTINUE READING