Protein helical structure determination using CD spectroscopy for solutions with strong background absorbance from 190 to 230nm.


Conventional empirical methods for the quantification of the helical content of proteins in solution using circular dichroism (CD) primarily rely on spectral data acquired between wavelengths of 190 and 230nm. The presence of chemical species in a protein solution with strong absorbance within this range can interfere with the ability to use these methods… (More)
DOI: 10.1016/j.bbapap.2014.10.001