Protein glycosylation analysis by HILIC-LC-MS of Proteinase K-generated N- and O-glycopeptides.

Abstract

Analysis of protein glycosylation is essential in order to correlate certain disease types with oligosaccharide structures on proteins. Here, a method for the MS characterization of site-specific protein glycosylation is presented. Using asialofetuin and fetuin as model substances, a protocol for glycopeptide dissection was developed based on unspecific… (More)
DOI: 10.1002/jssc.200900850

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