Protein-glycosphingolipid interactions revealed using catch-and-release mass spectrometry.

@article{Zhang2012ProteinglycosphingolipidIR,
  title={Protein-glycosphingolipid interactions revealed using catch-and-release mass spectrometry.},
  author={Yixuan Zhang and Lin Liu and Rambod Daneshfar and Elena N. Kitova and Caishun Li and Feng Jia and Christopher W. Cairo and John S Klassen},
  journal={Analytical chemistry},
  year={2012},
  volume={84 18},
  pages={
          7618-21
        }
}
Glycosphingolipids (GSL) on the surface of cells are important receptors in antigen/microbial recognition and cell adhesion. However, their functional characterization is often challenging. We have developed a catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay for the identification of specific interactions between water-soluble proteins or protein complexes with GSL incorporated into nanodiscs. The specificity and sensitivity of the assay is demonstrated for… CONTINUE READING

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