Protein-free and low-protein media for the cultivation of Leptospira.

  title={Protein-free and low-protein media for the cultivation of Leptospira.},
  author={Russell F. Bey and R. Colin Johnson},
  journal={Infection and immunity},
  volume={19 2},
A protein-free medium composed of charcoal-detoxified Tweens (polysorbates), vitamins B12 and B1, inorganic salts, and organic buffer is described that supports the growth and subculture of pathogenic and saprophytic Leptospira. Growth was initiated from small inocula, and cell densities of 10(9) organisms per ml were attained. Antigenicity and immunogenicity of Leptospira cultivated in this medium were similar to those of cells cultivated in serum-containing media. The protein-free medium was… CONTINUE READING

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