Protein fragment complementation in M.HhaI DNA methyltransferase.

  title={Protein fragment complementation in M.HhaI DNA methyltransferase.},
  author={Wonchae Choe and Srinivasan Chandrasegaran and Marc Ostermeier},
  journal={Biochemical and biophysical research communications},
  volume={334 4},
The 5mC DNA methyltransferase M.HhaI can be split into two individually inactive N- and C-terminal fragments that together can form an active enzyme in vivo capable of efficiently methylating DNA. This active fragment pair was identified by creating libraries of M.HhaI gene fragment pairs and then selecting for the pairs that code for an active 5mC methyltransferase. The site of bisection for successful protein fragment complementation in M.HhaI was in the variable region near the target… CONTINUE READING

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