Protein-fragment complementation assays (PCA) in small GTPase research and drug discovery.

@article{Westwick2006ProteinfragmentCA,
  title={Protein-fragment complementation assays (PCA) in small GTPase research and drug discovery.},
  author={John K. Westwick and Stephen W. Michnick},
  journal={Methods in enzymology},
  year={2006},
  volume={407},
  pages={388-401}
}
Small GTPases of the Ras and Rho families are among the most studied signaling proteins and represent promising therapeutic targets for human neoplastic disease. Despite the high level of interest in these proteins, direct analysis of most aspects of Ras protein biology in living cells has not been possible, because much of the details of Ras signaling cannot be studied in vitro but requires simple cell-based assays. Here we describe a strategy for directly analyzing Ras signaling pathways in… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Explore Further: Topics Discussed in This Paper

References

Publications referenced by this paper.
Showing 1-10 of 30 references

Capturing protein interactions in the secretory pathway of living cells.

Proceedings of the National Academy of Sciences of the United States of America • 2005

Chemical biology on PINs and NeeDLes.

Current opinion in chemical biology • 2005
View 1 Excerpt

Rational design and characterization of a Rac GTPase-specific small molecule inhibitor.

Proceedings of the National Academy of Sciences of the United States of America • 2004
View 1 Excerpt

modulator of protein kinase B/Akt

A. Montmarquette, S. W. Michnick
Mol. Cell. Biol • 2004

Similar Papers

Loading similar papers…