Protein factors required for in vitro transcription of Sendai virus genome.

@article{Mizumoto1995ProteinFR,
  title={Protein factors required for in vitro transcription of Sendai virus genome.},
  author={Kiyohisa Mizumoto and Koji Muroya and Takashi Takagi and T. Omata-Yamada and Hiroshi Shibuta and Kuniko Iwasaki},
  journal={Journal of biochemistry},
  year={1995},
  volume={117 3},
  pages={527-34}
}
To elucidate the mechanism of transcription and replication of Sendai virus, we developed an efficient and faithful in vitro transcription system using purified virus particles. The in vitro RNA synthesis was almost entirely dependent on the addition of eukaryotic cell extracts, including those from various cultured mammalian cells, mammalian tissues, and even from plant cells. The RNA products were almost identical to authentic mRNA species synthesized in the infected cells, in their size… CONTINUE READING