Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis.

Abstract

Sample preparation is crucial for extraction and higher resolution of proteins by two-dimensional gel electrophoresis (2-DE). In this study, we present an efficient protocol to extract proteins from mature rice leaves by minimizing the presence of nonprotein contaminants and by maximizing contact between the sample and extraction buffer. A combination of… (More)

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Cite this paper

@article{Islam2004ProteinEF, title={Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis.}, author={Nazrul Islam and Markus Nowak Lonsdale and Narayana M Upadhyaya and Thomas Higgins and H. Hirano and Ray J Akhurst}, journal={Proteomics}, year={2004}, volume={4 7}, pages={1903-8} }