Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis.

@article{Eman2006ProteinED,
  title={Protein expression dynamics during replicative senescence of endothelial cells studied by 2-D difference in-gel electrophoresis.},
  author={Michael R Eman and Elsa Regan-Klapisz and Martijn W. H. Pinkse and Inge M Koop and Johan Haverkamp and Albert J. R. Heck and Arie J. Verkleij and Jan Andries Post},
  journal={Electrophoresis},
  year={2006},
  volume={27 8},
  pages={1669-82}
}
Endothelial senescence contributes to endothelium dysfunctionality and is thereby linked to vascular aging. A dynamic proteomic study on human umbilical vein endothelial cells, isolated from three umbilical cords, was performed. The cells were cultured towards replicative senescence and whole cell lysates were subjected to 2-D difference gel electrophoresis… CONTINUE READING