Protein expression and secretion in the yeast Yarrowia lipolytica.

@article{Nicaud2002ProteinEA,
  title={Protein expression and secretion in the yeast Yarrowia lipolytica.},
  author={Jean-Marc Nicaud and Catherine Madzak and Peter J.M. van den Broek and Christof Gysler and Philippe Duboc and Peter Niederberger and C. Gaillardin},
  journal={FEMS yeast research},
  year={2002},
  volume={2 3},
  pages={371-9}
}
Strains and vectors for protein expression and secretion have been developed in the yeast Yarrowia lipolytica. Host strains were constructed with non-reverting auxotrophic markers, deletions of protease-encoding genes, and carrying a docking platform. To drive transcription, either the synthetic hp4d or the inducible POX2 promoter were used. Protein secretion is either directed by the targeting sequence of the alkaline extracellular protease or the extracellular lipase (LIP2p) signal sequence… CONTINUE READING