Protein expression and purification of human Zbtb7A in Pichia pastoris via gene codon optimization and synthesis.

Abstract

Human Zbtb7A was proved to be an important molecular switch in oncogenesis. However, it is difficult to obtain its protein expression in prokaryotic system, due to high G+C content and rare codons in zbtb7a gene. Therefore, to further research the function and application of this protein, we optimized its coding sequence according to the codon bias of… (More)
DOI: 10.1016/j.pep.2008.03.017

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