Protein encapsulation into biodegradable microspheres by a novel S/O/W emulsion method using poly(ethylene glycol) as a protein micronization adjuvant.

@article{Morita2000ProteinEI,
  title={Protein encapsulation into biodegradable microspheres by a novel S/O/W emulsion method using poly(ethylene glycol) as a protein micronization adjuvant.},
  author={Takahiro Morita and Y Sakamura and Yuji Horikiri and Takehiko Suzuki and Hiroyuki Yoshino},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2000},
  volume={69 3},
  pages={435-44}
}
A new method for preparing protein-loaded biodegradable microspheres by a process involving solid-in-oil-in-water (S/O/W) emulsion was established using poly(ethylene glycol) (PEG). In the first step, a protein solution was lyophilized with PEG, which resulted in the formation of spherical protein microparticles, less than 5 microm in diameter, dispersed in a continuous PEG phase. This process was well explained by the aqueous phase separation phenomenon induced by freezing-condensation. Since… CONTINUE READING

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