Protein carbonylation and aggregation precede neuronal apoptosis induced by partial glutathione depletion

@inproceedings{Dasgupta2012ProteinCA,
  title={Protein carbonylation and aggregation precede neuronal apoptosis induced by partial glutathione depletion},
  author={Anushka Dasgupta and Jianzheng Zheng and Oscar A Bizzozero},
  booktitle={ASN neuro},
  year={2012}
}
While the build-up of oxidized proteins within cells is believed to be toxic, there is currently no evidence linking protein carbonylation and cell death. In the present study, we show that incubation of nPC12 (neuron-like PC12) cells with 50 μM DEM (diethyl maleate) leads to a partial and transient depletion of glutathione (GSH). Concomitant with GSH disappearance there is increased accumulation of PCOs (protein carbonyls) and cell death (both by necrosis and apoptosis). Immunocytochemical… CONTINUE READING