Protein-carbohydrate interactions in human lysozyme probed by combining site-directed mutagenesis and affinity labeling.

Abstract

The synergism between apolar and polar interactions in the carbohydrate recognition by human lysozyme (HL) was probed by site-directed mutagenesis and affinity labeling. The three-dimensional structures of the Tyr63-->Leu mutant HL labeled with 2',3'-epoxypropyl beta-glycoside of N,N'-diacetylchitobiose (L63-HL/NAG-NAG-EPO complex) and the Asp102-->Glu… (More)

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Cite this paper

@article{Muraki2000ProteincarbohydrateII, title={Protein-carbohydrate interactions in human lysozyme probed by combining site-directed mutagenesis and affinity labeling.}, author={Michiro Muraki and Kazuaki Harata and Nami Sugita and K. I. Sato}, journal={Biochemistry}, year={2000}, volume={39 2}, pages={292-9} }