Protein body formation in leaves of Nicotiana benthamiana: a concentration-dependent mechanism influenced by the presence of fusion tags.

@article{Saberianfar2015ProteinBF,
  title={Protein body formation in leaves of Nicotiana benthamiana: a concentration-dependent mechanism influenced by the presence of fusion tags.},
  author={Reza Saberianfar and Jussi Joonas Joensuu and Andrew Jonathan Conley and Rima Menassa},
  journal={Plant biotechnology journal},
  year={2015},
  volume={13 7},
  pages={
          927-37
        }
}
Protein bodies (PBs) are endoplasmic reticulum (ER) derived organelles originally found in seeds whose function is to accumulate seed storage proteins. It has been shown that PB formation is not limited to seeds and green fluorescent protein (GFP) fused to either elastin-like polypeptide (ELP) or hydrophobin (HFBI) fusion tags induce the formation of PBs in leaves of N. benthamiana. In this study, we compared the ELP- and HFBI-induced PBs and showed that ELP-induced PBs are larger than HFBI… CONTINUE READING

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