Protein assay for heme oxygenase-1 (HO-1) induced by chemicals in HepG2 cells.

@article{Miyamoto2009ProteinAF,
  title={Protein assay for heme oxygenase-1 (HO-1) induced by chemicals in HepG2 cells.},
  author={Yohei Miyamoto and Keiyu Ohshida and Kaori Sasago},
  journal={The Journal of toxicological sciences},
  year={2009},
  volume={34 6},
  pages={709-14}
}
Levels of heme oxygenase-1 (HO-1), a stress response protein, were measured to examine oxidative stress induced by several chemicals in HepG2 cells with and without S9mix using an ELISA. CdCl(2), heme, and diclofenac sodium salt (diclofenac) were used as inducers of HO-1. Acetaminophen (AAP) and cyclophosphamide (CP) were used as oxidative stress inducers. Stannic mesoporphyrin (SnMP) was used as an inhibitor of HO activity. Cytotoxicity was determined, and HO-1 levels were measured in HepG2… CONTINUE READING