Protein and proteome phosphorylation stoichiometry analysis by element mass spectrometry.

@article{Krueger2006ProteinAP,
  title={Protein and proteome phosphorylation stoichiometry analysis by element mass spectrometry.},
  author={Ralf Krueger and Dieter K{\"u}bler and Roser Palliss{\'e} and Andreas Burkovski and Wolf D. Lehmann},
  journal={Analytical chemistry},
  year={2006},
  volume={78 6},
  pages={1987-94}
}
Protein phosphorylation stoichiometry was assessed by two analytical strategies. Both are based on element mass spectrometry (ICPMS, inductively coupled plasma mass spectrometry) and simultaneous monitoring of (31)P and (34)S. One strategy employs a combination of 1D gel electrophoresis, in-gel digestion, and final microLC-ICPMS analysis (microLC = capillary liquid chromatography). The other strategy uses the combination of 1D gel electrophoresis, protein blotting, and imLA-ICPMS (imLA… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.