Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains.

@article{Kaspar2010ProteinAO,
  title={Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains.},
  author={Stephanie Kaspar and Diana Weier and Winfriede Weschke and Hans P Mock and Andrea Matros},
  journal={Analytical and bioanalytical chemistry},
  year={2010},
  volume={398 7-8},
  pages={2883-93}
}
Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mass spectrometry. The majority of the ~160 proteins identified were involved in translation, protein synthesis, or protein destination. The NP proteome was enriched for stress defense molecules, while… CONTINUE READING

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