Protein Structures Forming the Shell of Primitive Bacterial Organelles

@article{Kerfeld2005ProteinSF,
  title={Protein Structures Forming the Shell of Primitive Bacterial Organelles},
  author={C. Kerfeld and M. Sawaya and Shiho Tanaka and C. V. Nguyen and M. Phillips and M. Beeby and T. Yeates},
  journal={Science},
  year={2005},
  volume={309},
  pages={936 - 938}
}
Bacterial microcompartments are primitive organelles composed entirely of protein subunits. Genomic sequence databases reveal the widespread occurrence of microcompartments across diverse microbes. The prototypical bacterial microcompartment is the carboxysome, a protein shell for sequestering carbon fixation reactions. We report three-dimensional crystal structures of multiple carboxysome shell proteins, revealing a hexameric unit as the basic microcompartment building block and showing how… Expand
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This review updates the current understanding of bacterial microcompartment shell proteins, providing new insights and highlighting outstanding questions. Expand
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Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway
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The results demonstrate that, unlike membrane-bound organelles of eukaryotes, in carboxysomes the interior of the compartment forms first, at a distinct site within the cell, and it is proposed that the principles of car boxysome assembly that are uncovered extend to diverse bacterial microcompartments. Expand
Atomic-Level Models of the Bacterial Carboxysome Shell
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Two proteins of previously unknown function assemble to form pentameric structures whose size and shape are compatible with formation of vertices in an icosahedral shell, and combining these pentamers with the hexamers previously elucidated gives two plausible, preliminary atomic models for the carboxysome shell. Expand
Protein-based organelles in bacteria: carboxysomes and related microcompartments
TLDR
The carboxysome, a prototypical bacterial microcompartment that is found in cyanobacteria and some chemoautotrophs, encapsulates ribulose-1,5-bisphosphate carboxylase/oxygenase and carbonic anhydrase, and thereby enhances carbon fixation by elevating the levels of CO2 in the vicinity of RuBisCO. Expand
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The crystal structure of an intact shell from Haliangium ochraceum is presented, revealing the basic principles of bacterial microcompartment shell construction and can inform the design and engineering of shells with new functionalities. Expand
Bacterial microcompartments insights into the structure, mechanism, and engineering applications.
  • S. Tsai, T. Yeates
  • Biology, Medicine
  • Progress in molecular biology and translational science
  • 2011
TLDR
Crystal structures of the individual shell proteins have revealed details about how they self-assemble and how pores through their centers facilitate molecular transport into and out of the microcompartments. Expand
Bacterial microcompartments moving into a synthetic biological world.
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The molecular structure of bacterial microcompartments is examined and the potential to engineer these intriguing organelles for biotechnological applications is explored. Expand
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