Protein Structures Forming the Shell of Primitive Bacterial Organelles

@article{Kerfeld2005ProteinSF,
  title={Protein Structures Forming the Shell of Primitive Bacterial Organelles},
  author={Cheryl A. Kerfeld and Michael R. Sawaya and Shiho Tanaka and C. V. Nguyen and Martin L. Phillips and Morgan Beeby and Todd O. Yeates},
  journal={Science},
  year={2005},
  volume={309},
  pages={936 - 938}
}
Bacterial microcompartments are primitive organelles composed entirely of protein subunits. Genomic sequence databases reveal the widespread occurrence of microcompartments across diverse microbes. The prototypical bacterial microcompartment is the carboxysome, a protein shell for sequestering carbon fixation reactions. We report three-dimensional crystal structures of multiple carboxysome shell proteins, revealing a hexameric unit as the basic microcompartment building block and showing how… 
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References

SHOWING 1-10 OF 29 REFERENCES
Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra
TLDR
This review evaluates the evidence that relates carboxysome structure to function in the carbon metabolism of autotrophic prokaryotes and examines similarities to newly discovered particles found in heterotrophs.
A HEX-1 crystal lattice required for Woronin body function in Neurospora crassa
TLDR
The crystal structure of HEX-1 is solved at 1.8 Å, which provides the structural basis of its self-assembly and reveals the existence of three intermolecular interfaces that promote the formation of a three-dimensional protein lattice.
THE CARBOXYSOMES (POLYHEDRAL BODIES) OF AUTOTROPHIC PROKARYGTES
1. Polyhedral bodies are present in several groups of autotrophic bacteria that assimilate inorganic carbon via the Calvin cycle, including members of the colourless sulphur‐ oxidizing bacteria,…
Minor proteins, mobile arms and membrane–capsid interactions in the bacteriophage PRD1 capsid
TLDR
Multiresolution models of the PRD1 capsid provide fine details of the molecular interactions associated with particle stability and membrane dynamics and newly revealed proteins suggest how the metastable vertex works and how the capsid edges are stabilized.
The carboxysome, a prokaryotic organelle: a mini-review
TLDR
The structure, composition, and function of carboxysomes are studied with great interest and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is demonstrated to be associated with these prokaryotic inclusions.
L-A virus at 3.4 Å resolution reveals particle architecture and mRNA decapping mechanism
TLDR
The structure of the yeast L-A virus is determined by X-ray crystallography and reveals a trench at the active site of the decapping reaction and suggests a role for nearby residues in the reaction.
The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.
TLDR
The crystal structure of the large ribosomal subunit from Haloarcula marismortui is determined at 2.4 angstrom resolution, and it includes 2833 of the subunit's 3045 nucleotides and 27 of its 31 proteins.
A Novel Evolutionary Lineage of Carbonic Anhydrase (ε Class) Is a Component of the Carboxysome Shell
TLDR
It is reported that the carboxysomal shell protein, CsoS3, from Halothiobacillus neapolitanus is a novel carbonic anhydrase (epsilon-class CA) that has an evolutionary lineage distinct from those previously recognized in animals, plants, and other prokaryotes.
Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core
TLDR
To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X‐ray crystallography, demonstrating that nucleotides also bind to the outer core layer, providing a substrate sink.
Functional Organelles in Prokaryotes: Polyhedral Inclusions (Carboxysomes) of Thiobacillus neapolitanus
The polyhedral inclusions of Thiobacillus neapolitanus have been isolated; they contain ribulose diphosphate carboxylase.
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