Protein Profiling of the Medicinal Leech Salivary Gland Secretion by Proteomic Analytical Methods

  title={Protein Profiling of the Medicinal Leech Salivary Gland Secretion by Proteomic Analytical Methods},
  author={Isolda P. Baskova and L. L. Zavalova and A. V. Basanova and Sergei A. Moshkovskii and Victor G. Zgoda},
  journal={Biochemistry (Moscow)},
Protein diversity of the high molecular weight fraction (molecular mass > 500 daltons) of salivary grand secretion of the medicinal leech Hirudo medicinalis has been demonstrated using methods of proteomic analysis. One-dimensional (1D) electrophoresis revealed the presence of more than 60 bands corresponding to molecular masses ranging from 11 to 483 kD. 2D-electrophoresis revealed more than 100 specific protein spots differing in molecular masses and pI values. SELDI-mass spectrometry… 
Proteomic analysis methods for characterization of proteins from the salivary gland secretions of the medicinal leech during different seasons
Findings suggest a high content of newly revealed proteins in SGS of medicinal leech correlates with multiple positive clinical effects of hirudotherapy realized through SGS, but the mechanisms of these effects remain unclear.
Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis
Comparison of detected molecular masses with those of six known biologically active compounds produced by medicinal leeches revealed their uneven distribution in SGS of each of the three leech species, which opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies.
Protein-lipid particles of medicinal leech salivary gland secretion; Their size and morphology
SGS, preliminary frozen at −70°C, transformed into a multitude of similar small particles visualized by transmission electron microscopy, whose fixed distribution resembled water crystal structure.
Lysozyme activity of the salivary gland secretion of the medicinal leeches H. verbana, H. medicinalis, and H. orientalis
It is suggested that lysozyme activity of the leech secretions is determined not only by 5 isoforms of destabilase-lysozyme, but by some other enzymes which can utilize these substrates including ly sozymes other than i-type (invertebrate) lysozymes.
Novel possibilities in the study of the salivary proteomic profile using SELDI-TOF/MS technology.
In the present study, surface-enhanced laser desorption/ionization-time-of-flight/mass spectrometry (SELDI-TOF/MS), one of the most recent proteomic tools for the identification of novel biomarkers, is reviewed and the possible use of this technique in salivary proteomic studies is discussed.
May Salivary Gland Secretory Proteins from Hematophagous Leeches (Hirudo verbana) Reach Pharmacologically Relevant Concentrations in the Vertebrate Host?
Analysis of protein extracts of unfed or fed leeches by 2D electrophoresis calculated the relative molar amounts of individual salivary proteins in the mass range of 17–60 kDa which may be released from a single leech during feeding, and found concentrations seem sufficiently high to exert biochemical interactions with target molecules in the host.
Diverse molecular data demonstrate that commercially available medicinal leeches are not Hirudo medicinalis
It is shown, with mitochondrial sequences and nuclear microsatellites, that there are at least three species of European medicinal leech, and that leeches marketed as H. medicinalis are actually Hirudo verbana.
Characterization and optimization of lyophilization and storage conditions of Leech saliva extract from the tropical leech Hirudinaria manillensis.
Investigation of leech saliva extract from the medicinal Malaysian leech Hirudinaria manillensis showed that storage temperature drastically affected the biological activity of LSE with -20 °C as the optimum temperature and samples stored at ambient temperature and +4 °C were light photosensitive and adversely affected when stored in polypropylene tubes.
Be ready at any time: postprandial synthesis of salivary proteins in salivary gland cells of the haematophagous leech Hirudo verbana
Leeches (Hirudo verbana) refill their salivary gland reservoirs very soon after a blood meal to readily prepare for another act of feeding, confirming that haematophagous leeches are opportunistic ectoparasites.


Separation of Monomerizing and Lysozyme Activities of Destabilase from Medicinal Leech Salivary Gland Secretion
A new technique for preparation of a true leech secretion (secretion-I) is elaborated, characterized by the complete absence of the leech intestinal channel contents and has no proteolytic activity.
Multiple forms of medicinal leech destabilase-lysozyme.
Proteomic Technologies in Modern Biomedical Science
Methods of solubilization, electrophoresis, chromatographic protein separation, and visualization of protein spots in gels are described, and modern methods of mass spectrometry used in proteomic studies include combination of protein chips with mass spectra.
A Carboxypeptidase Inhibitor from the Medical Leech Hirudo medicinalis
Circular dichroism and nuclear magnetic resonance spectroscopy analysis indicate that recombinant LCI is a compactly folded globular protein, stable to a wide range of pH and denaturing conditions.
Isolation and characterization of hirustasin, an antistasin-type serine-proteinase inhibitor from the medical leech Hirudo medicinalis.
Despite the high similarity to antistasin, particularly in the vicinity of the putative reactive-site peptide bond, hirustasin neither inhibits blood coagulation in vitro nor amidolytic activity of isolated factor Xa, suggesting structural elements other than the reactive site sequence significantly influence the specificity of antistasIn-type proteinase inhibitors.
Bdellastasin, a serine protease inhibitor of the antistasin family from the medical leech (Hirudo medicinalis)--primary structure, expression in yeast, and characterisation of native and recombinant inhibitor.
Comparison by mass spectroscopy, far-ultraviolet circular dichroism studies, sequence determination, and inhibition characteristics demonstrated the identity of recombinant and native bdellastasin.
The Three-dimensional Structure of Recombinant Leech-derived Tryptase Inhibitor in Complex with Trypsin
The x-ray crystal structure of recombinant leech-derived tryptase inhibitor (rLDTI) has been solved and a series of rLDTI variants constructed to probe this hypothesis confirmed that the amino-terminal Lys-Lys sequence plays a role in inhibition of human lungtryptase but not of trypsin or chymotrypsin.
An isopeptide bond splitting enzyme from Hirudo medicinalis similar to gamma-glutamyl transpeptidase.
A new enzyme from Hirudo medicinalis capable of splitting gamma-glutamyl-p-nitroanilide and Glu--Lys-(N6-gamma-glutamyllysine) (isopeptidic bond between the epsilon-amino group of lysine and the
Tryptase: a mast cell serine protease.