Protein-Polyelectrolyte Phase Boundaries

@inproceedings{Mattison2001ProteinPolyelectrolytePB,
  title={Protein-Polyelectrolyte Phase Boundaries},
  author={Kevin W. Mattison and Isabelle J. Brittain and Paul L Dubin},
  year={2001}
}
The separation of proteins by polyelectrolyte coacervation or precipitation is based on electrostatically-driven complex formation. We have investigated complexation between the globular protein BSA and the polyelectrolyte poly(dimethyldially1ammonium chloride) (PDMDAAC) using light-scattering techniques to monitor solution turbidity. Turbidimetric pH titrations were used to determine the specific pH values where soluble complex formation is initiated (pH,) and where phase separation occurs (pH… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 32 references

Stoichiometry and the Mechanism of Complex Formation in Protein - Polyelectrolyte Coacervation

  • L. Ahmed, J. Xia, L. P. Dubin, E. Kokufuta
  • J . Macro . Sci . - Pure Appl . Chem .
  • 1994

Critical Conditions for the Binding of Polyelectrolytes to Small Oppositely Charged Mixed Micelles

  • G. Manning, D. W. McQuigg, J. I. Kaplan, P. L. Dubin
  • J . Phys . Chem .
  • 1992

Effects of Protein Charge Heterogeneity in Protein - Polyelectrolyte Complexation

  • J. M. Park, B. B. Muhoberac, P. L. Dubin, J. Xia
  • Macromolecules
  • 1992

Complex Coacervates Involving Protein Biopolymer Pairs

  • A. Veis
  • Polym . Prepr . ( Am . Chem . SOC . , Diu . Polym…
  • 1991

Separation of Proteins by Ultrafiltration

  • A. G. Bozzano, G. Andrea, C. E. Glatz
  • J . Membr . Sci .
  • 1991

Patch Controlled Attractive Electrostatic Interactions between Similarly Charged Proteins and Adsorbents

  • V. Lesins, E. Ruckenstein
  • Colloid Polym . Sci .
  • 1988

Similar Papers

Loading similar papers…