Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies.

@article{kerstrm1985ProteinGA,
  title={Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies.},
  author={Bo {\AA}kerstr{\"o}m and Thomas Brodin and Km Reis and Lars Bj{\"o}rck},
  journal={Journal of immunology},
  year={1985},
  volume={135 4},
  pages={2589-92}
}
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein recently isolated from group G streptococci. We have investigated the avidity of protein G for various monoclonal and polyclonal Ig of the IgG class, and compared it with the binding properties of protein A, the staphylococcal Fc-binding protein. Radiolabeled Ig were mixed with Sepharose-coupled protein G or protein A, and the amounts of radioactivity bound to the matrix-coupled bacterial proteins were determined. The… CONTINUE READING

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