Protein Engineering of Chit42 Towards Improvement of Chitinase and Antifungal Activities

  title={Protein Engineering of Chit42 Towards Improvement of Chitinase and Antifungal Activities},
  author={Mojegan Kowsari and M. R. Motallebi and M. Reza Zamani},
  journal={Current Microbiology},
The antagonism of Trichoderma strains usually correlates with the secretion of fungal cell wall degrading enzymes such as chitinases. Chitinase Chit42 is believed to play an important role in the biocontrol activity of Trichoderma strains as a biocontrol agent against phytopathogenic fungi. Chit42 lacks a chitin-binding domain (ChBD) which is involved in its binding activity to insoluble chitin. In this study, a chimeric chitinase with improved enzyme activity was produced by fusing a ChBD from… CONTINUE READING

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