Protein Degradation Rate in Arabidopsis thaliana Leaf Growth and Development.

@article{Li2017ProteinDR,
  title={Protein Degradation Rate in Arabidopsis thaliana Leaf Growth and Development.},
  author={Lei Li and Clark J. Nelson and Josua Tr{\"o}sch and Ian R. Castleden and Shaobai Huang and A. Harvey Millar},
  journal={The Plant cell},
  year={2017},
  volume={29 2},
  pages={
          207-228
        }
}
We applied 15N labeling approaches to leaves of the Arabidopsis thaliana rosette to characterize their protein degradation rate and understand its determinants. The progressive labeling of new peptides with 15N and measuring the decrease in the abundance of >60,000 existing peptides over time allowed us to define the degradation rate of 1228 proteins in vivo. We show that Arabidopsis protein half-lives vary from several hours to several months based on the exponential constant of the decay rate… CONTINUE READING
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