Protein B23 (M.W./pI = 37 kD/5.1) is the only major protein extracted from HeLa nucleoli with 3M urea.

Abstract

HeLa nucleoli were isolated using the NP-40 method and subsequently extracted with 3M urea. The extract was incubated at 60 degrees C for 30 min, and precipitated proteins were removed by centrifugation. The supernatant was analyzed by one- and two-dimensional SDS polyacrylamide gel electrophoresis (PAGE). Protein B23 was the only major protein extracted… (More)

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@article{Yung1992ProteinB, title={Protein B23 (M.W./pI = 37 kD/5.1) is the only major protein extracted from HeLa nucleoli with 3M urea.}, author={Benjamin Y-M. Yung and Eric K-W Hui and P. K. Chan}, journal={Life sciences}, year={1992}, volume={51 12}, pages={915-20} }