Corpus ID: 18784868

Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites.

@article{Riggs1997ProtectiveMA,
  title={Protective monoclonal antibody defines a circumsporozoite-like glycoprotein exoantigen of Cryptosporidium parvum sporozoites and merozoites.},
  author={M. W. Riggs and A. L. Stone and P. Yount and R. Langer and M. Arrowood and D. Bentley},
  journal={Journal of immunology},
  year={1997},
  volume={158 4},
  pages={
          1787-95
        }
}
The apicomplexan protozoan parasite Cryptosporidium parvum causes a diarrheal disease in humans and other mammals for which specific therapy and immunoprophylaxis are unavailable. Passive immunization with Abs against whole C. parvum organisms has variable efficacy in immunocompromised or neonatal hosts. Because apical and surface-exposed zoite Ags of the Apicomplexa are critical to infectivity and targets of protective immunity, we examined the ability of mAbs generated against such Ags in C… Expand
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Role of Immunoglobulin A Monoclonal Antibodies against P23 in Controlling Murine Cryptosporidium parvum Infection
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It is concluded that IgA MAbs directed to neutralization-sensitive P23 epitopes may have utility in passive immunization against murine C. parvum infection. Expand
Cryptosporidium parvum Sporozoite Pellicle Antigen Recognized by a Neutralizing Monoclonal Antibody Is a β-Mannosylated Glycolipid
ABSTRACT The protozoan parasite Cryptosporidium parvum is an important cause of diarrhea in humans, calves, and other mammals worldwide. No approved vaccines or parasite-specific drugs are currentlyExpand
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TLDR
The surface and apical localization of these glycoproteins and the neutralizing effect of the MAb and α-N-acetylgalactosamine-specific lectins strongly implicate these proteins and their glycotopes as playing a role in C. parvum-host cell interactions. Expand
Phospholipases and Cationic Peptides Inhibit Cryptosporidium parvum Sporozoite Infectivity by Parasiticidal and Non-Parasiticidal Mechanisms
TLDR
Collective observations suggest that cationic peptides and phospholipases neutralize C. parvum by mechanisms that are predominantly either parasiticidal or non-parasiticidal, respectively. Expand
Characterization and Formulation of Multiple Epitope-Specific Neutralizing Monoclonal Antibodies for Passive Immunization against Cryptosporidiosis
TLDR
Findings indicate that polyvalent neutralizing MAb formulations targeting epitopes on defined antigens may provide optimal passive immunization against cryptosporidiosis. Expand
Cryptosporidium parvum gp40/15 Is Associated with the Parasitophorous Vacuole Membrane and Is a Potential Vaccine Target
TLDR
Indirect immunofluorescence assay showed that Cpgp40/15 is associated with the parasitophorous vacuole membrane (PVM) during intracellular development and both anti-gp40/ 15 and anti- gp40 antibodies demonstrated the ability to neutralize C. parvum infection in vitro. Expand
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References

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Neutralizing Monoclonal Antibody Protects Against Cryptosporidium parvum Infection by Inhibiting Sporozoite Attachment and Invasion
TLDR
Because CSL is mechanistically involved in the CSP-like reaction, it was purified for further study of its potential role in the infection process, and sporozoite attachment and invasion assays were performed in vitro, allowing examination of sporozoites-host cell interactions not readily studied in vivo. Expand
Localization of parasite antigens in Cryptosporidium parvum-infected epithelial cells using monoclonal antibodies.
An immunogold ultrastructural study was made of Cryptosporidium parvum-infected intestinal cells from SCID mice to locate parasite antigens recognized by monoclonal antibodies raised againstExpand
Infectivity and neutralization of Cryptosporidium parvum sporozoites
TLDR
When isolated sporozoites were incubated with heat-inactivated immune bovine serum, 25 times the 50% infective dose for 7-day-old mice was completely neutralized and sporozoite infectivity in vivo by direct intraintestinal injection of isolated sporzoites in 7- day-old BALB/c mice. Expand
Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro.
TLDR
Inhibition of attachment and entry were found to be related to the antibody titer against the authentic circumsporozoite protein on the sporozoite surface, especially when peptides were administered with alum or complete Freund's adjuvant. Expand
Malaria sporozoites release circumsporozoite protein from their apical end and translocate it along their surface.
TLDR
It is proposed that CS protein released from the apical end, the leading end of gliding sporozoites, adheres to the sporozoite surface and is translocated posteriorly by a cytochalasin-sensitive and apparently actin-mediated surface motor, which drives gliding motility. Expand
Evaluation of an animal model system for cryptosporidiosis: therapeutic efficacy of paromomycin and hyperimmune bovine colostrum-immunoglobulin.
TLDR
An animal model system in which scid mice were used to screen drugs for inhibitory activity against C. parvum and hyperimmune bovine colostrum-immunoglobulin was developed, finding that paromomycin treatment was very effective against villus surface infections in suckling mice and considerably less effective against infections in inaccessible sites such as abscessed crypts and stomach pits seen in weaned and adultScid mice. Expand
Hyperimmune bovine colostrum neutralizes Cryptosporidium sporozoites and protects mice against oocyst challenge.
TLDR
Activity of colostral whey, produced by a cow immunized with oocysts of Cryptosporidium parvum and found to provide prophylaxis against cryptosporidiosis in calves, was tested in 2 experiments and it was determined that protection was mediated by specific anti-sporozoite activity. Expand
Dual action of anti-sporozoite antibodies in vitro.
TLDR
These results confirm previous reports of the extended action of antibodies on Plasmodium falciparum after entering hepatocytes by producing a strong intrahepatocyte inhibitory effect in addition to the inhibitory effects on sporozoite entry. Expand
Rapid clearance of malaria circumsporozoite protein (CS) by hepatocytes
TLDR
It is found that intravenously injected CS constructs bind rapidly to the basolateral surface of hepatocytes, provided that the recombinant proteins contain region II-plus, and that they are aggregated. Expand
Multiple oral inoculations with Cryptosporidium parvum as a means of immunization for production of monoclonal antibodies.
TLDR
Oral immunization of suckling mice with Cryptosporidium parvum results in a humoral response to a limited set of antigens and is able to select for monoclonal antibodies that predominantly recognize sporozoite surface and apical complex antIGens. Expand
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