Protective effect of vitamin A against the methotrexate-induced damage to small intestine: a study on the crypt cells.

  title={Protective effect of vitamin A against the methotrexate-induced damage to small intestine: a study on the crypt cells.},
  author={Y Kosakai and Toshiharu Horie and Shoji Awazu},
  journal={Pharmacology \& toxicology},
  volume={69 4},
Vitamin A (VA) protects the small intestine from the methotrexate (MTX)-induced damage. The in vivo effects of MTX and/or VA on crypt cells of small intestine were investigated using rats orally administered MTX (15 mg/kg body weight) and/or VA (5,000 IU/kg body weight). The thymidine kinase activity of crypt cells separated from villus cells of small intestine of MTX plus VA-treated rats was lower than that from control rats but higher than that from MTX-treated rats. VA-treated rats showed… 
Effect of Vitamin A against Methotrexate-Induced Damage to the Small Intestine in Rats
The results confirmed that administration of VA decreased the MTX-induced damage to the small intestine, and this protective effect of VA may have clinical applications in cancer chemotherapy.
Protective effect of a synthetic analog of prostaglandin E(1) on the small intestinal damage induced by the administration of methotrexate to rats.
Results show that OP-1206 alleviates the methotrexate-induced damage to the small intestine of rats, using in vitro everted intestine and in situ intestinal loop techniques.
Amelioration of methotrexate-induced malabsorption by vitamin A
It is found that the coadministration of vitamin A (VA) with MTX protected the small intestine from MTX-induced damage, and it seems likely that VA ameliorates MTx-induced malabsorption.
Effect of vitamin A on methotrexate cytotoxicity in L1210 murine leukemia cells in culture
The present study proved in L1210 murine leukemia cells in vitro that VA did not disturb the antitumor activity of MTX and this depression was not affected by the presence of VA.
Alleviation by garlic of antitumor drug-induced damage to the intestine.
Results suggest that AGE may protect the small intestine of rats from antitumour drug-induced damage, and may even protect against damage from aged garlic extract.
Vitamin A deficiency exacerbates methotrexate-induced jejunal injury in rats.
The interaction of vitamin A deficiency and small intestinal injury may explain the efficacy ofitamin A supplementation in preventing childhood diarrheal disease mortality in developing countries, and highlights the need for ensuring adequate vitamin A status in people worldwide with diseases and/or treatments which may injure the gastrointestinal tract.
Potent protective effect of apricot and beta-carotene on methotrexate-induced intestinal oxidative damage in rats.
  • N. Vardı, H. Parlakpınar, Ali Otlu
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2008
Vitamin A, a useful biochemical modulator capable of preventing intestinal damage during methotrexate treatment.
Biochemical modulation used to prevent methotrexate-induced malabsorption by vitamin A coadministration will be of great use in metotrexate cancer chemotherapy.
A study on the possible role of mitochondrial damage reactive nitrogen species and reactive oxygen species in the pathogenesis of methotrexate induced small intestinal damage in the rat
It is concluded that MTX causes acute inflammatory changes with damage to the villi and crypts of lieberkhun that may result in decreased nutrient absorption associated with barrier and absorptive dysfunction of the small intestine.


Vitamin A protects the small intestine from methotrexate-induced damage in rats.
It has been shown histologically, biochemically, physicochemical, physicochemically and physiologically that VA protects the small intestine from the damage induced by MTX.
Effect of methotrexate on drug absorption from the rat small intestine in situ and in vitro.
  • V. Venho
  • Medicine, Chemistry
    Acta pharmacologica et toxicologica
  • 1976
The methotrexate-induced reversible decrease in absorption seems to be attributable at least partly to diminished water flux through the intestinal wall, although other mechanisms may also exist.
The cytotoxicity of methotrexate in mouse small intestine in relation to inhibition of folic acid reductase and of DNA synthesis.
In spite of the early inhibition of DNA synthesis, methotrexate induces relatively few degenerating cells in the proliferative crypt epithelium during the first 6 hr, and it is concluded that inhibition ofDNA synthesis by metotrexate in mouse intestinal crypts is not selective but is balanced by concurrent inhibition of RNA or of protein or of both.
Changes in the mucosa of the small intestine following methotrexate administration or abdominal x-irradiation.
  • G. Altmann
  • Medicine
    The American journal of anatomy
  • 1974
Adult male rats received 5 mg methotrexate daily and were sacrificed 1, 1.5, 2, 2.5 and 3 days after the beginning of the treatment. Other groups received 9,000 rads of abdominal x-radiation and were
Change in small intestinal brush border membranes of rats following methotrexate administration.
An apparent increase in the electronegative charge of brush border membrane vesicles per unit of membrane protein following methotrexate administration may possibly cause the change in fluorescence spectra and polarization of safranin 0.1.