Protective effect of lipoic acid on micronuclei induction by cyclophosphamide

  title={Protective effect of lipoic acid on micronuclei induction by cyclophosphamide},
  author={Elangovan Selvakumar and Chidambaram Prahalathan and Periyasamy Thandavan Sudharsan and P. Varalakshmi},
  journal={Archives of Toxicology},
The present study investigated the protective efficacy of DL-α-lipoic acid on the cyclophosphamide (CP)-induced clastogenicity using the in vivo micronucleus assay. Male Wistar rats of 140 ± 20 g were categorized into eight groups. Five groups were administered CP (40 mg/kg body weight, intraperitonealy) to induce genotoxicity; four of these groups received a single intraperitoneal injection of lipoic acid at a dose of either 100 or 200 mg/kg body weight, and either 30 or 60 min prior to CP… 


This study revealed that mice MN assay can be used as a genotoxicological test-system since some methodological particularities were observed.

Evaluation of mutagenic and anti-mutagenic potential of alpha-lipoic acid by chromosomal aberration assay in mice

ALA has a dose and time-dependent protective effect against the mutagenicity induced by CP and has no mutagenic effect on mouse bone marrow cells.

Antigenotoxic effect of lipoic acid against mitomycin-C in human lymphocyte cultures

Results demonstrated anticlastogenic and antimutagenic effects of LA against MMC induced genotoxicity and are encouraging that LA can be a possible chemopreventive agent in tumorigenesis in both cancer patients and in health care persons handling anti-cancer drugs.

Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats

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Influence of novel naphthalimide-based organoselenium on genotoxicity induced by an alkylating agent: the role of reactive oxygen species and selenoenzymes

The protective effect of these compounds may be primarily associated with the improvement of the activity of antioxidant and detoxifying enzymes that are known to protect the DNA and other cellular components from oxidative damage.

Protective Effects of Ruitn and / or Hesperidin Against Doxorubicin-Induced Hepatotoxicity

It can be concluded that natural plant components such as Rutin and Hesperidin could protect the liver against DXR-induced liver toxicity.



In vivo anticlastogenic effects of L-ascorbic acid in mice.

The problem of oncostatic specificity of cyclophosphamide (NSC-26271): Studies on reactions that control the alkylating and cytotoxic activity.

It could be demonstrated by the reaction of 4-hydroxy-CP with thiols yielding 4-(S-R)-mercapto CP derivatives that the toxication of 3-carbon unit of CP can be controlled under physiologic conditions of pH and temperature and the significance of these findings to the problem of CP specificity is discussed.

Antimutagenic effects of stobadine: review of results.

Metabolic differences between whole blood and isolated lymphocyte cultures for micronucleus (MN) induction by cyclophosphamide and benzo[a]pyrene.

In isolated lymphocyte cultures supplemented with S9, CP and B[a]P induced a statistically significant increase in MN which was not observed in whole blood cultures, however, the direct-acting agent methyl methanesulphonate showed an increase inMN frequency in a dose-dependent manner in both culture methods.

Clinical Pharmacokinetics of Cyclophosphamide

Clinical correlation between kinetic data and efficacy and/or toxicity awaits studies evaluating the time course of specific cytotoxic metabolites, and conflicting data have been obtained in several animal studies.

Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization

Flow cytometric analysis of freshly prepared human peripheral blood lymphocytes reveals that lipoic acid acts mainly to normalize a subpopulation of cells severely compromised in thiol status rather than to increase thiol content beyond physiological levels, suggesting it may have clinical relevance in restoration of severely glutathione deficient cells.

Historical vehicle and positive control micronucleus data in mice and rats.