Protective effect of N‐acetyl‐serotonin on the nonenzymatic lipid peroxidation in rat testicular microsomes and mitochondria

  title={Protective effect of N‐acetyl‐serotonin on the nonenzymatic lipid peroxidation in rat testicular microsomes and mitochondria},
  author={Mariana Gavazza and {\'A}ngel Maquieira Catala},
  journal={Journal of Pineal Research},
Abstract:  N‐acetyl‐serotonin, the immediate precursor of melatonin in the tryptophan metabolic pathway in the pineal gland, has been reported to be an antioxidant. The aim of this study was to test the in vitro protective effect of N‐acetyl‐serotonin on the ascorbate‐Fe++ induced lipid peroxidation of polyunsaturated fatty acids (PUFAs) located in testis microsomes and mitochondria. We assayed increasing concentrations (0–10 mm) of N‐acetyl‐serotonin in testis microsomes and (0–1 mm) of N… 

Protective effect of melatonin on ascorbate‐Fe2+ lipid peroxidation of polyunsaturated fatty acids in rat liver, kidney and brain microsomes: a chemiluminescence study

Results indicate a selective protection of C20:4 n6 and C22:6 n3 by melatonin during non‐enzymatic lipid peroxidation of rat liver, kidney and brain microsomes.

Protective effects of melatonin and N‐acetylserotonin on aflatoxin B1‐induced lipid peroxidation in rats

Melatonin can be considered as a protective pharmacological agent in intoxication with AFB1 and the protective effect of NAc‐5HT against aflatoxin‐induced LPO broadens the knowledge about its antioxidative properties.

N-Acetyl Serotonin Alleviates Oxidative Damage by Activating Nuclear Factor Erythroid 2-Related Factor 2 Signaling in Porcine Enterocytes

It is demonstrated that NAS attenuated oxidative stress-induced cellular injury in porcine enterocytes by regulating Nrf2 signaling, providing new insights into a functional role of NAS in maintaining intestinal homeostasis.

N-acetylserotonin reduces lipopolysaccharide-induced lipid peroxidation in vitro more effectively than melatonin.

The results of the reported study clearly indicate that N-acetylserotonin is a much stronger antioxidant in vitro than melatonin in terms of reducing oxidative damage to lipid membranes, but it remains still unclear how the features relate to in vivo circumstances.

N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide

N-Acetyl-serotonin protected the HepG2 cells from apoptosis through regulating the mitochondrial apoptosis pathway, involving inhibition of mitochondrial hyperpolarization, release of mitochondrial apoptogenic factors, and caspase activity.

Inverse correlation between endogenous melatonin levels and oxidative damage in some tissues of SAM P8 mice

The results show that high levels of melatonin in spleen and thymus correlate with lower protein and lipid damage, and suggest that a treatment with this molecule might help to reduce age‐associated functional deficits in many organs, including those of the immune system.

Protective Effect of N-Acetylserotonin against Acute Hepatic Ischemia-Reperfusion Injury in Mice

The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation, which demonstrates that NAS ameliorates hepatic IR injury.

An overview of lipid peroxidation with emphasis in outer segments of photoreceptors and the chemiluminescence assay.

  • Á. Catala
  • Biology, Chemistry
    The international journal of biochemistry & cell biology
  • 2006

Antioxidant systems and oxidative stress in the testes

The testes contain an elaborate array of antioxidant enzymes and free radical scavengers to ensure that the twin spermatogenic and steroidogenic functions of this organ are not impacted by oxidative stress.



Characterization of the antioxidant effects of melatonin and related indoleamines in vitro

An antiperoxidative and antioxidant action of melatonin which is devoid of pro‐oxidant effect on non‐lipid substrates is supported.

N-acetylserotonin suppresses hepatic microsomal membrane rigidity associated with lipid peroxidation.

Relative efficacies of indole antioxidants in reducing autoxidation and iron‐induced lipid peroxidation in hamster testes

Melatonin and related indoles at pharmacological concentrations protect against both the autoxidation of lipids as well as induced peroxidationof lipids in testes.

N‐acetyl‐serotonin (normelatonin) and melatonin protect neurons against oxidative challenges and suppress the activity of the transcription factor NF‐κB

Normelatonin's and melatonin's neuroprotective activity in SK‐N‐MC neuroblastoma cells may be mediated by the suppression of NF‐κB activation, and normelatonin was more potent in the suppressing of the activation of NF-κB by H2O2 in SK-N-MC cells compared to melatonin, no apparent differences in the extent of suppression could be detected in primary neurons.

Pharmacological aspects of N‐acetyl‐5‐methoxytryptamine (melatonin) and 6‐methoxy‐ 1,2,3,4‐tetrahydro‐β‐carboline (pinoline) as antioxidants: Reduction of oxidative damage in brain region homogenates

The ability of pinoline to protect against H2O2‐induced lipid peroxidation of different rat brain homogenates was investigated and evidence for either synergistic, additive, or antagonistic interactions between melatonin and pinoline was found.

Effect of melatonin and metabolites on copper-mediated oxidation of flow density lipoprotein.

The present results indicate that the pineal hormone melatonin appears to have little antiatherogenic property as regards the oxidation of LDL, however, its main breakdown product 6-hydroxymelatonin inhibits LDL-oxidation comparable to vitamin E.

N-acetyl-serotonin reduces copper (I) ion-induced lipid peroxidation in bovine retinal homogenates.

A pharmacological potential of N-acetyl-serotonin combating free radical oxidative damages in the ocular tissues is supported, which is only 29% as effective as vitamin E in suppressing the copper (I)-induced lipid peroxidation.

Inhibition of lipid peroxidation by N-acetylserotonin and its role in retinal physiology.

N-Acetylserotonin reduces the rate of ABAP-initiated lipid peroxidation in a concentration-dependent manner, and it is suggested that NAS acts as a physiological antioxidant in retinal photoreceptor cells.

Melatonin and tryptophan derivatives as free radical scavengers and antioxidants.

Melatonin crosses all morphophysiological barriers and enters equally well all cells in the organism, and its antioxidative actions include the protection of lipids in the cell membrane, proteins in the cytosol, and DNA in the nucleus.