Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions.

@article{Israeli1993ProtectionOF,
  title={Protection of freeze-dried Escherichia coli by trehalose upon exposure to environmental conditions.},
  author={Ella Israeli and Brenda T. Shaffer and Bruce Lighthart},
  journal={Cryobiology},
  year={1993},
  volume={30 5},
  pages={
          519-23
        }
}
Freeze-dried cultures of wild-type and genetically engineered strains of Escherichia coli lost their colony-forming ability upon exposure to air, visible light, and certain relative humidity levels. Both strains could be maximally protected from these lethal effects with 100 mM trehalose, a concentration calculated to just saturate the interphospholipid spaces in the cell membrane, thus preserving the liquid-crystalline structure. The trehalose protection was observed for at least 96 h… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 15 CITATIONS

Preserving Biological Materials in Protective Polymers

VIEW 8 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Practice and prospects of microbial preservation.

  • FEMS microbiology letters
  • 2013
VIEW 1 EXCERPT
CITES BACKGROUND

Preservation of micro-organisms by drying; a review.

  • Journal of microbiological methods
  • 2006
VIEW 2 EXCERPTS
CITES BACKGROUND