Protection of flounder against hirame rhabdovirus (HIRRV) with a DNA vaccine containing the glycoprotein gene.

Hirame rhabdovirus (HIRRV) is an important virus of cultured flounder (Paralichthys olivaceus). We tested the protective immunogenicity of DNA-based vaccines against this virus. Genes encoding the nucleocapsid protein (N) and the C-terminal half of the glycoprotein (G) were amplified by RT-PCR and separately cloned into the eukaryotic expression vector… (More)