Protection of epithelial function in human jejunum cultured with hydrocortisone.

  title={Protection of epithelial function in human jejunum cultured with hydrocortisone.},
  author={J. Scott and Timothy J. Peters},
  journal={The American journal of physiology},
  volume={244 5},
Over 24-h culture with hydrocortisone (400 nM), activity of brush-border alkaline phosphatase, alpha-glucosidase, and leucyl-2-naphthylamidase and cytoplasmic-mitochondrial malate dehydrogenase increased (P less than 0.05) by 80-133% compared with controls. Uptake of 3-O-methyl-D-[14C]glucose after 24-h culture was increased (P less than 0.05) by 30% compared with cultures without hydrocortisone. Labeling of protein with L-[14C]tyrosine and glycoprotein with D-[3H]glucosamine increased (P less… Expand
Hydrocortisone-induced DNA endoreplication in human trabecular cells in vitro.
Cultures which had been exposed to hydrocortisone showed a significant shift toward the higher DNA classes, in contrast to the untreated control cultures, and the relevance of these findings to the disease glaucoma in man is discussed. Expand
Organ culture of rabbit ileum as a model for the investigation of the mechanism of intestinal damage by enteropathogenic Escherichia coli.
Organ culture of rabbit ileum has been established as a model for the investigation of the mechanism of intestinal damage by enteropathogenic Escherichia coli (EPEC). Loops of rabbit ileum wereExpand
Ontogenic changes in secretory component expression by villous and crypt cells of rat small intestine.
Major changes in the ability of the immature crypt cell to produce the specific receptor for transepithelial transport of polymeric immunoglobulins occur during the fourth week of rat life. Expand
Small intestinal mucosa changes, including epithelial cell proliferative activity, of children receiving total parenteral nutrition (TPN)
The small intestinal histology disaccharidase activities as well as the incorporation of [3H]thymidine into DNA of biopsies maintained in organ culture from seven children receiving total parenteral nutrition (TPN) are in general agreement with the hypoplastic effect of TPN in animals. Expand
Effect of acute and chronic glucocorticoid treatments on epithelial cell proliferation in the esophagus and small intestine of rats
It is suggested that acute glucocorticoid treatment nonsignificantly reduces the number of cells in the cell cycle in the esophageal epithelium, whereas chronic treatment increases thenumber of proliferating cells in both esphageal and jejunal crypt epithelia. Expand
Effects of enteropathogenic Escherichia coli on microvillar membrane proteins during organ culture of rabbit intestinal mucosa.
Findings suggest that enteropathogenic E. coli accelerate the normal shedding of microVillar membrane proteins as vesicles, and may stimulate a compensatory increase in microvillar membrane protein synthesis. Expand
The Effects of Steroids Upon the Gastrointestinal Tract
In man, peptic ulcer disease is not clearly associated with glucocorticoid therapy, but exacerbation of subclinical intestinal infections and perforative lesions have been observed in both animals and man given glucOCorticoids. Expand
Influences of dexamethasone on the maturation of fetal mouse intestinal mucosa in organ culture.
  • J. Beaulieu, R. Calvert
  • Biology, Medicine
  • Comparative biochemistry and physiology. A, Comparative physiology
  • 1985
Results indicate that glucocorticoids influence directly the differentiation of absorptive cells and goblet cells in the small intestine during the fetal period. Expand
Intestinal tissue and cell cultures.
Primary cell cultures which allow maintenance of a more physiological environment for the epithelial cells like contacts with their in vivo counterparts, mesenchymal cells or extracellular matrix molecules, have proved to be promising approaches. Expand