Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

@article{He2008ProtectionOG,
  title={Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine},
  author={Hanjiang He and Wei Wang and Zhongdao Wu and Zhiyue Lv and Jun Li and Lizhi Tan},
  journal={Cellular and Molecular Immunology},
  year={2008},
  volume={5},
  pages={385-391}
}
In this study, the full lipL21 gene fragment encoding outer membrane protein LipL21 was cloned from L. interrogans serovar Lai and inserted into eukaryotic expression vector pcDNA3.1(+). The guinea pigs were immunized with pcDNA3.1(+)-lipL21, pcDNA3.1(+) or PBS. Six weeks after the second immunization, the splenocytes were isolated to detect their proliferative ability by lymphocyte transformation experiments. In addition, microscopic agglutination test was used for quantitative detection of… CONTINUE READING
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