Protection of Chinese hamster ovary cells from hyperthermic killing by cycloheximide or puromycin.

@article{Lee1986ProtectionOC,
  title={Protection of Chinese hamster ovary cells from hyperthermic killing by cycloheximide or puromycin.},
  author={Young Jun Lee and William C. Dewey},
  journal={Radiation research},
  year={1986},
  volume={106 1},
  pages={98-110}
}
Cycloheximide (CHM) and puromycin (PUR) were used at various concentrations up to maxima of 10 micrograms/ml and 100 micrograms/ml, respectively, which inhibited protein synthesis by 95% without any cytotoxicity. The drugs were added to the cells for a maximum period of 7 h, with various combinations for treatment before, during, and after heating. Maximum protection, i.e., a 10,000-fold increase in survival from 5 X 10(-6) to 5 X 10(-2) after 4 h at 43 degrees C, required both 1-2 h of… CONTINUE READING

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Hyperthermia: malformations to chaperones.

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