Protection and recycling of alpha-tocopherol in human erythrocytes by intracellular ascorbic acid.

@article{May1998ProtectionAR,
  title={Protection and recycling of alpha-tocopherol in human erythrocytes by intracellular ascorbic acid.},
  author={James Michael May and Zhenhuan Qu and Seema Mendiratta},
  journal={Archives of biochemistry and biophysics},
  year={1998},
  volume={349 2},
  pages={281-9}
}
Ascorbic acid can recycle alpha-tocopherol from the tocopheroxyl free radical in lipid bilayers and in micelles, but such recycling has not been demonstrated to occur across cell membranes. In this work the ability of intracellular ascorbate to protect and to recycle alpha-tocopherol in intact human erythrocytes and erythrocyte ghosts was investigated. In erythrocytes that were 80% depleted of intracellular ascorbate by treatment with the nitroxide Tempol, both 2,2'-azobis(2-amidinopropane… CONTINUE READING