Protection against mycoplasma infection using expression-library immunization

@article{Barry1995ProtectionAM,
  title={Protection against mycoplasma infection using expression-library immunization},
  author={Michael A. Barry and Wayne C. Lai and Stephen A. Johnston},
  journal={Nature},
  year={1995},
  volume={377},
  pages={632-635}
}
AS is evident from the human immunodeficiency virus epidemic, there is no systematic method for producing a vaccine. Genetic immunization1is a new approach to vaccine production that has many of the advantages of live/attenuated pathogens but no risk of infection. It involves introducing DNA encoding a pathogen protein into host cells and has shown promise in several disease models2á¤-13. Here we describe a new method for vaccine development, expression-library immunization, which makes use of… Expand
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The main advantage of ELI is twofold; it provides a rapid screening protocol for an entire genome and the readout of screening is often protection, an end goal for vaccine development. Expand
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Summary:  Genetic immunization is a novel method for vaccination in which DNA is delivered into the host to drive both cellular and humoral immune responses against its protein product. While geneticExpand
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It is suggested that expression libraries generated from viral DNA can produce the immunologic advantages of live vaccines without risk of reversion to pathogenic viruses. Expand
Immunization of mice with a Mycobacterium tuberculosis genomic expression library results in lower bacterial load in lungs after challenge with BCG.
TLDR
Results suggest that the genomic expression library contains genes encoding proteins that are protective against M. tuberculosis infection. Expand
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TLDR
Adoptive transfer of protection from vaccinated or infected mice into naive mice by transfer of purified T cells and clones shows that protection is expressed by antigen-specific cytotoxic T cells that produce interferon-γ and lyse infected macrophages. Expand
Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B.
TLDR
It is reported that few rounds of library screening allow identification of protective pools of defined antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Expand
Protection against leishmaniasis by DNA injection of a plasmid encoding the Leishmania major Cpn60 gene
TLDR
In this study a eukaryotic expression vector, pcDNA3.1 encoding the L. major chaperonin 60 (Cpn60) was constructed and tested in vitro to confirm that the mammalian translational machinery could synthesize the protein. Expand
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Some of the approaches being evaluated to improve responsiveness in large animals including the use of cytokines as adjuvants, targeting molecules as delivery ligands, electroporation and CpG oligonucleotides are discussed. Expand
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