Proteasome activity profiling: a simple, robust and versatile method revealing subunit-selective inhibitors and cytoplasmic, defense-induced proteasome activities.

@article{Gu2010ProteasomeAP,
  title={Proteasome activity profiling: a simple, robust and versatile method revealing subunit-selective inhibitors and cytoplasmic, defense-induced proteasome activities.},
  author={C. Gu and I. Kołodziejek and Johana C. Misas-Villamil and T. Shindo and T. Colby and M. Verdoes and K. Richau and J. Schmidt and H. Overkleeft and R. V. D. van der Hoorn},
  journal={The Plant journal : for cell and molecular biology},
  year={2010},
  volume={62 1},
  pages={
          160-70
        }
}
The proteasome plays essential roles in nearly all biological processes in plant defense and development, yet simple methods for displaying proteasome activities in extracts and living tissues are not available to plant science. Here, we introduce an easy and robust method to simultaneously display the activities of all three catalytic proteasome subunits in plant extracts or living plant tissues. The method is based on a membrane-permeable, small-molecule fluorescent probe that irreversibly… Expand
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