Proteasome activity and its relationship with protein phosphorylation during capacitation and acrosome reaction in human spermatozoa.

@article{Morales2007ProteasomeAA,
  title={Proteasome activity and its relationship with protein phosphorylation during capacitation and acrosome reaction in human spermatozoa.},
  author={Patricio Morales and E. Santana Diaz and Milene Kong},
  journal={Society of Reproduction and Fertility supplement},
  year={2007},
  volume={65},
  pages={269-73}
}
We have shown that the proteasome is present in mammalian sperm and plays a role during fertilisation. In this work we studied the relationship between protein phosphorylation and proteasomal activity in human sperm. Aliquots of motile sperm were incubated for 0, 5 and 18 h at 37 degrees C, 5% CO2, with different concentration of the kinase inhibitors genistein, H89 or tamoxifen. Control aliquots were treated with the inhibitor solvent. The chymotrypsin-like activity of the proteasome was… CONTINUE READING
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