Protease-activated kinase II as the potential mediator of insulin-stimulated phosphorylation of ribosomal protein S6.

@article{Perisic1983ProteaseactivatedKI,
  title={Protease-activated kinase II as the potential mediator of insulin-stimulated phosphorylation of ribosomal protein S6.},
  author={Olga Perisic and Jolinda A. Traugh},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 16},
  pages={9589-92}
}
One of the earliest responses to insulin in target cells is stimulation of the phosphorylation of ribosomal protein S6. When exponentially growing 3T3-L1 cells are serum-starved, little phosphorylation of S6 is observed; however, following addition of insulin (10(-7) M), up to 5 phosphoryl groups are incorporated into S6. An enzyme mediating the insulin-stimulated phosphorylation of S6 has been identified as protease-activated kinase II. Two-dimensional peptide maps of tryptic digests of S6… CONTINUE READING