Protease B of Saccharomyces cerevisiae: isolation and regulation of the PRB1 structural gene.

@article{Moehle1987ProteaseBO,
  title={Protease B of Saccharomyces cerevisiae: isolation and regulation of the PRB1 structural gene.},
  author={C M Moehle and M W Aynardi and M R Kolodny and F J Park and Elizabeth W. Jones},
  journal={Genetics},
  year={1987},
  volume={115 2},
  pages={255-63}
}
We have isolated the structural gene, PRB1, for the vacuolar protease B of Saccharomyces cerevisiae from a genomic library by complementation of the prb1-1122 mutation. Deletion analysis localized the complementing activity to a 3.2-kilobase pair XhoI-HindIII restriction enzyme fragment. The fragment was used to identify a 2.3-kilobase mRNA. S1 endonuclease mapping indicated that the mRNA and the gene were colinear. No introns were detected. The mRNA is of sufficient size to encode a protein of… CONTINUE READING

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