Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis.

@article{Alderson2006ProtamineSP,
  title={Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis.},
  author={Jon Alderson and Ben P. Wilson and G{\"o}tz Laible and Peter Pfeffer and Phil J. L'Huillier},
  journal={Animal reproduction science},
  year={2006},
  volume={91 1-2},
  pages={23-30}
}
The present study investigated whether protamine sulfate can be used to improve the efficiency of bovine sperm mediated transgenesis (SMT) by protecting the plasmid pCX-EGFP against nuclease activity. A high proportion (31%) of bovine spermatozoa transfected with the plasmid pCX-EGFP maintain their motility. Using an in vitro assay, protamine sulfate protected the plasmid against degradation by DNase I. However, upon transfecting spermatozoa, the plasmid remained intact regardless of whether it… CONTINUE READING