Prostaglandin specific binding in hamster myometrial low speed supernatant.

  title={Prostaglandin specific binding in hamster myometrial low speed supernatant.},
  author={F. A. Kimball and L J Wyngarden},
  volume={9 3},
A charcoal adsorption method was developed to measure specific prostaglandin binding in low speed supernates of hamster myometrial homogenates. This method was used to characterize and quantitate PGE-1-specific binding. The equilibrium binding constants and the concentration of specific PGE-1 binding sites were determined during the hamster estrous cycle. The apparent association constant for 12 different preparations was 1.16 plus or minus 0.08 times 10-9M-1. The concentration of PGE-1… CONTINUE READING