Corpus ID: 21978274

Prostaglandin and fatty acid omega- and (omega-1)-oxidation in rabbit lung. Acetylenic fatty acid mechanism-based inactivators as specific inhibitors.

@article{Muerhoff1989ProstaglandinAF,
  title={Prostaglandin and fatty acid omega- and (omega-1)-oxidation in rabbit lung. Acetylenic fatty acid mechanism-based inactivators as specific inhibitors.},
  author={A. Muerhoff and D. Williams and N. Reich and C. Cajacob and P. Ortiz de Montellano and B. Masters},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 2},
  pages={
          749-56
        }
}
Terminal acetylenic fatty acid mechanism-based inhibitors (Ortiz de Montellano, P. R., and Reich, N. O. (1984) J. Biol. Chem. 259, 4136-4141) were used as probes in determining the substrate specificity of rabbit lung cytochrome P-450 isozymes of pregnant animals in both microsomes and reconstituted systems. Lung microsomal and reconstituted P-450 form 5-catalyzed lauric acid omega- and (omega-1)-hydroxylase activities were inhibited by a 12-carbon terminal acetylenic fatty acid, 11-dodecynoic… Expand
Involvement of cytochrome P450 2E1 in the (omega-1)-hydroxylation of oleic acid in human and rat liver microsomes.
TLDR
It can be ascertained that P450 2E1 is the main enzyme involved in the (omega-1)-hydroxylation of oleic acid, which was shown to be mainly catalyzed by P450 4A enzymes in human liver microsomes. Expand
Cytochrome P 450-Derived Arachidonic Acid Metabolism in the Rat Kidney : Characterization of Selective Inhibitors 1
We characterized the inhibitory activity of several acetylenic and olefinic compounds on cytochrome P450 (CYP)-derived arachidonic acid v-hydroxylation and epoxidation using rat renal corticalExpand
[omega]-Hydroxylation of Oleic Acid in Vicia sativa Microsomes (Inhibition by Substrate Analogs and Inactivation by Terminal Acetylenes)
TLDR
Results suggest that distinct monooxygenases catalyze [omega]-hydroxylation of medium- and long-chain fatty acids in V. sativa microsomes and inhibition of oleic acid was competitive with linoleic acid but noncompetitive with lauric acid. Expand
Kinetic profile of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-specific inhibitors.
TLDR
CYP4A1, despite its low expression in extrahepatic tissues, may constitute the major source of 20-HETE synthesis and the ability of CYP4A2 and -4A3 to catalyze the formation of two opposing biologically active metabolites, 20- HETE and 11, 12-epoxyeicosatrienoic acid, may be of great significance to the regulation of vascular tone. Expand
Involvement of cytochrome P 450 2 E 1 in the ( v – 1 )-hydroxylation of oleic acid in human and rat liver microsomes
In vitro techniques have been used to investigate the nature of microsomal cytochrome P450 involved in the metabolism of oleic acid, a physiological monounsaturated fatty acid. Like lauric acid,Expand
Kinetic profile of the rat CYP4A isoforms: arachidonic acid metabolism and isoform-specific inhibitors.
TLDR
It is suggested that CYP4A1, despite its low expression in extrahepatic tissues, may constitute the major source of 20-HETE synthesis and may be of great significance to the regulation of vascular tone. Expand
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Abstract Pyrazole, acetone, and ethanol are known to induce cytochrome P450 2E1 (CYP 2 E1) and fatty acid (co-l)-hydroxylation in rat liver microsomes. However, the nature of the P450 enzyme involvedExpand
REGULATION AND INHIBITION OF ARACHIDONIC ACID ω-HYDROXYLASES AND 20-HETE FORMATION
TLDR
The substrate selectivity and expression of arachidonic acid omega-hydroxylases, regulation of these enzymes during disease, and the application of enzyme inhibitors to study 20-HETE function are discussed. Expand
Positional Specificity of Rabbit CYP4B1 for ω-Hydroxylation1of Short-Medium Chain Fatty Acids and Hydrocarbons
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Cytochrome P450-dependent desaturation of lauric acid: isoform selectivity and mechanism of formation of 11-dodecenoic acid.
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Non-competitive, intermolecular isotope effect experiments demonstrated further that CYP4B1-mediated terminal desaturation of DDA is initiated by removal of a hydrogen atom from the omega-1 rather than the omega position. Expand
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References

Cytochrome P-450: Structure, Mechanism, and Biochemistry
TLDR
The Cytochrome P450 and the Metabolism and Bioactivation of Arachidonic Acid and Eicosanoids in Plants and the Diversity and Importance of Microbial Cytochromes P450 are studied. Expand