Prostaglandin E2 binding and cyclic AMP production in isolated bone cells

@article{Dziak2006ProstaglandinEB,
  title={Prostaglandin E2 binding and cyclic AMP production in isolated bone cells},
  author={R. Dziak and Douglas Hurd and Katelyn Miyasaki and M J Brown and N. Weinfeld and Ernest Hausmann},
  journal={Calcified Tissue International},
  year={2006},
  volume={35},
  pages={243-249}
}
SummaryThe binding of prostaglandin E2 (PGE2) to bone cells was studied to provide direct evidence for the existence of specific receptors in bone. Bone cells were isolated by collagenase digestion of fetal and newborn rat calvaria. Isolated cells were incubated with3H-PGE2 and collected on Millipore filters. Specific binding was determined by subtracting the binding that occurred with 10−6 M non-radioactive PGE2 and3H-PGE2 from that with3H-PGE2 alone. With heterogeneous cell preparations and… 

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