Prospects of androgenetic induction in Lupinus spp.

@article{Skrzypek2008ProspectsOA,
  title={Prospects of androgenetic induction in Lupinus spp.},
  author={Edyta Skrzypek and Ilona Mieczysława Czyczyło-Mysza and Izabela Marcińska and Maria Wędzony},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2008},
  volume={94},
  pages={131-137}
}
Anthers cultures of six Polish cultivars of pasture lupin (Lupinus L.) were examined for their androgenic response. Anthers with microspores at the uninucleate stage were isolated from flower buds and cultured in liquid media. Better viability of androgenetic structures was obtained when donor plants had grown under field as opposed to greenhouse conditions. A density of five anthers per 0.5 ml medium was more conducive to androgenetic induction than 25 anthers per 0.5 ml medium. Addition of 5… 
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Haploids in flowering plants: origins and exploitation.
  • J. Dunwell
  • Biology, Medicine
    Plant biotechnology journal
  • 2010
TLDR
The range of techniques available for the isolation or induction of haploids are described and their value in a range of areas are discussed, from fundamental research on mutant isolation and transformation, through to applied aspects of quantitative genetics and plant breeding.
Anther culture for haploid and doubled haploid production
  • M. Germanà
  • Biology
    Plant Cell, Tissue and Organ Culture (PCTOC)
  • 2010
TLDR
This review article deals with the current status of knowledge on the production of haploids and DHs through pollen embryogenesis and, in particular, anther culture.
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